Plant Transcription Factor Database
Previous version: v3.0
Brassica rapa
SBP Family
Species TF ID Description
XP_009101792.1SBP family protein
XP_009101793.1SBP family protein
XP_009102983.1SBP family protein
XP_009102984.1SBP family protein
XP_009102985.1SBP family protein
XP_009102986.1SBP family protein
XP_009104082.1SBP family protein
XP_009105462.1SBP family protein
XP_009105463.1SBP family protein
XP_009106325.1SBP family protein
XP_009109814.1SBP family protein
XP_009109815.1SBP family protein
XP_009109816.1SBP family protein
XP_009113768.1SBP family protein
XP_009113770.1SBP family protein
XP_009113771.1SBP family protein
XP_009113772.1SBP family protein
XP_009113774.1SBP family protein
XP_009113775.1SBP family protein
XP_009114006.1SBP family protein
XP_009114007.1SBP family protein
XP_009114008.1SBP family protein
XP_009115660.1SBP family protein
XP_009118853.1SBP family protein
XP_009119750.1SBP family protein
XP_009120939.1SBP family protein
XP_009120940.1SBP family protein
XP_009124484.1SBP family protein
XP_009124485.1SBP family protein
XP_009126287.1SBP family protein
XP_009127706.1SBP family protein
XP_009128184.1SBP family protein
XP_009128185.1SBP family protein
XP_009132698.1SBP family protein
XP_009132699.1SBP family protein
XP_009132700.1SBP family protein
XP_009132954.1SBP family protein
XP_009139002.1SBP family protein
XP_009139003.1SBP family protein
XP_009141347.1SBP family protein
XP_009142046.1SBP family protein
XP_009142812.1SBP family protein
XP_009143829.1SBP family protein
XP_009144795.1SBP family protein
XP_009146272.1SBP family protein
XP_009147622.1SBP family protein
XP_009147623.1SBP family protein
XP_009147624.1SBP family protein
XP_009149579.1SBP family protein
SBP Family Introduction

SQUAMOSA promoter binding proteins (SBPs) form a major family of plant-specific transcription factors related to flower development. Although SBPs are heterogeneous in primary structure, they share a highly conserved DNA-binding domain (DBD) that has been suggested to be zinc binding. Here we report the NMR solution structures of DBDs of two SBPs of Arabidopsis thaliana, SPL4 and SPL7. The two share essentially the same structural features. Each structure contains two zinc-binding sites consisting of eight Cys or His residues in a Cys3HisCys2HisCys or Cys6HisCys sequence motif in which the first four residues coordinate to one zinc and the last four coordinate to the other. These structures are dissimilar to other known zinc-binding structures, and thus represent a novel type of zinc-binding motif. The electrostatic profile on the surface suggested that a continuous region, including all the conserved basic residues, is involved in the DNA binding, the mode of which is likely to be novel as well.

Yamasaki K, Kigawa T, Inoue M, Tateno M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Nunokawa E, Ishizuka Y, Terada T, Shirouzu M, Osanai T, Tanaka A, Seki M, Shinozaki K, Yokoyama S.
A novel zinc-binding motif revealed by solution structures of DNA-binding domains of Arabidopsis SBP-family transcription factors
J Mol Biol. 2004 Mar 12;337(1):49-63.
PMID: 15001351

SBP-box genes were first characterized as SQUAMOSA promoter binding proteins (SBPs) to regulate the expression of MADS-box genes in early flower development of A. majus (Klein et al., 1996). Since then, SBP-box genes have been identified in many plants including green alga, moss, silver birch, A. majus, Arabidopsis and maize. They play critical roles in regulating flower and fruit development as well as other physiological processes ([Moreno et al., 1997],[Eriksson et al., 2004], [Lannenpaa et al., 2004], [Arazi et al., 2005] and [Kropat et al., 2005]). It has been reported that Arabidopsis SPL3, SPL8 and SPL14 involves in flowering, sporogenesis, GA signaling and toxin resistance ([Cardon et al., 1997], [Unte et al., 2003], [Stone et al., 2005] and [Zhang et al., 2006]) while maize tga1 and tomato LeSPL-CNR affect fruit development ([Wang et al., 2005a] and [Manning et al., 2006]).

SBP-box genes encode proteins sharing a conserved DNA-binding domain of 79 amino acid residues. It has been proved that the DNA-binding domain of SBP-box genes is necessary and sufficient to bind to a palindromic GTAC core motif ([Klein et al., 1996], [Cardon et al., 1997], [Cardon et al., 1999], [Lannenpaa et al., 2004] and [Birkenbihl et al., 2005]). Studies on the NMR solution structure of the fragment of Arabidopsis SPL4 and SPL7 revealed that the DNA-binding domain of SBPs consisted of two separate zinc-binding sites. One zinc finger is C3H or C4 and the other is C2HC (Yamasaki et al., 2004 K. Yamasaki et al.,).

Guo AY, Zhu QH, Gu X, Ge S, Yang J, Luo J.
Genome-wide identification and evolutionary analysis of the plant specific SBP-box transcription factor family.
Gene. 2008 Jul 15;418(1-2):1-8.
PMID: 18495384